| 학회 | 한국화학공학회 |
| 학술대회 | 2022년 봄 (04/20 ~ 04/23, 제주국제컨벤션센터) |
| 권호 | 28권 1호, p.914 |
| 발표분야 | [주제 10] 헬스케어 |
| 제목 | Selective metal sequestration through E. coli using zinc finger proteins |
| 초록 | The major transcriptional and translational regulators in eukaryotes and prokaryotes are zinc finger (ZF) proteins. The structural and functional aspects of these proteins have been intensively investigated, owing to their crucial importance in the Central Dogma. ZF proteins have a distinct feature as metalloproteins, because the specific secondary folding in local ZF domains is generated in the presence of zinc ions. This study demonstrates the possible application of zinc fingers for recycling of metal ions, to obtain selective cobalt ions using induced E. coli systems. A classical three-zinc finger domain (PARIS_ZF2-4) from the protein expressed in the brain, PARIS, was investigated to understand metal coordination. The results provided the valuable information that PARIS_ZF2-4 selectively coordinates to cobalt in the E. coli system. Iron and copper cannot replace metal ions in PARIS, although iron improves the expression level of zinc-bound PARIS_ZF2-4. In addition, PARIS_ZF2-4 was expressed and purified for basic characterization and showed cobalt-binding abilities. |
| 저자 | 황윤하1, 이승재2 |
| 소속 | 1Department of Chemistry, 2Jeonbuk National Univ. |
| 키워드 | 생물화공(Biochemical Engineering) |
| 원문파일 | 초록 보기 |
