| 학회 |
한국공업화학회 |
| 학술대회 |
2014년 가을 (11/12 ~ 11/14, 엑스코(대구)) |
| 권호 |
18권 2호 |
| 발표분야 |
나노-포스터 |
| 제목 |
Self-assembled DNA nanostructures prepared by rolling cirlce amplification for siRNA delivery |
| 초록 |
A large-scale production of DNA nanostructures for siRNA delivery has been achieved by an isothermal enzymatic process of rolling circle amplification (RCA). Our system allowed the preparation of more than 200-fold amplified DNA products using ss circular DNA template. Y-shaped DNA nanostructures with sticky overhangs, which can hybridize with siRNAs, were generated through the programmed molecular self-assembly. The DNA nanostructures loaded with folate conjugated-siRNAs showed enhanced cellular uptake as well as dose-dependent gene silencing. |
| 저자 |
장보라, 홍철암, 이혁진
|
| 소속 |
이화여자대 |
| 키워드 |
RCA; nanostructure; siRNA delivery
|
| E-Mail |
|
