| 초록 |
In this study, we were investigated difference insight into the microbial composition and structure of granule samples obtained from full-scale (F1) and sequential two stage lab-scale (R1 and R2) UASB reactors. In addition, as results of microbial community analysis, we were designed a specific oligonuleotide probe of five bacteria species and of three archaeal species. And then, we were investigated by the fluorescence in situ hybridization (FISH) technique combined with confocal laser scanning microscopy (CLSM) to visualize the locations of above mentioned microorganisms of particular interest in full-scale (F1) and sequential two stage lab-scale (R1 and R2) UASB reactors. As a results, syntrophus sp. was the most abundant type of bacteria in the all of granules. These granules contained mainly Proteobacteria. In archaeal communities, acetoclastic methanogens are more abundant than hydrogenotrophic methanogens. Quantification using fluorescence in situ hybridization showed that Methanosaeta was predominant (83 to 95% of the total active archaea) in all reactor granules. |
