| 초록 |
In this study, we present development of electrospun silk fibroin nanofibrous scaffolds with two-stage hydroxyapatite (HA) particles for bone tissue engineering using human adipose-derived stem cells (hADSCs). This scaffolds were prepared by incorporating the HA particles inside the scaffolds and then depositing the HA particles on top of the mussel adhesive motif-coated scaffolds. The use of the silk scaffolds with two-stage HA particles substantially enhanced osteogenic differentiation and mineralization of hADSCs in vitro. Ultimately, transplantation of hADSCs using the developed scaffolds resulted in significantly promoted bone formation in a critical-sized calvarial bone defect model. Therefore, our study demonstrates the applicability of the silk scaffolds with two-stage HA particles for efficient bone tissue engineering. This study was supported by a grant (NRF-2013R1A1A2A10061422) funded by the National Research Foundation (NRF) of Korea, Republic of Korea. |
