| 초록 |
Temperature-sensitive plasmid of Mannheimia succiniciproducens MBEL55E was generated from the chemical mutagenesis of plasmid pMVSCS1, which is a native plasmid obtained from Mannheimia varigena. The Ts plasmid in M. succiniciproducens was fully functional at 30°C, but failed to replicate above 42°C. The temperature sensitive plasmid was further modified to generate markerless mutants combined with Cre/loxP system. This result confers that multiple gene deletion in M. succiniciproducens can be applied to generate an enhanced succinic acid producing strain. [This work was supported by the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government (MOST) (No. 2005-01294). Further supports by the LG Chem Chair Professorship, IBM SUR program, and by the KOSEF through the Center for Ultramicrochemical Process Systems are appreciated]. |
