| 학회 |
한국공업화학회 |
| 학술대회 |
2017년 봄 (05/10 ~ 05/12, 광주 김대중컨벤센센터(Kimdaejung Convention Center)) |
| 권호 |
21권 1호 |
| 발표분야 |
나노 |
| 제목 |
Nanoplasmonic system of RNA folding and assembly during pre-mRNA splicing |
| 초록 |
RNA splicing plays important roles in transcriptome and proteome diversity. Herein, we describe the use of a nanoplasmonic system that unveils RNA folding and assembly during pre-mRNA splicing wherein the quantification of mRNA splice variants is not taken into account. With a couple of SERS-probes and plasmonic probes binding at the boundary sites of exon-2/intron-2 and intron-2/exon-3 of the pre-mature RNA of the β-globin gene, the splicing process brings the probes into the plasmonic bands. For plasmonic probes, a plasmon shift increase of ∼29 nm is measured by plasmonic coupling. The increased scattering intensity and SERS fingerprinting reveal the clear dynamics of premRNA splicing. Moreover, a time-resolved experiment of individual RNA molecules exhibited a successful splicing and an inhibited splicing event by 33 μM biflavonoid isoginkgetin, a general inhibitor of RNA splicing. The results show that the RNA splicing is successfully monitored with the nanoplasmonic system.
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| 저자 |
유병선, 송소진, Anh H. Nguyen, 심상준
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| 소속 |
고려대 |
| 키워드 |
RNA splicing; Nanoplasmonic system; Plasmonic coupling; Raman scattering (SERS)
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| E-Mail |
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