화학공학소재연구정보센터
학회 한국공업화학회
학술대회 2014년 가을 (11/12 ~ 11/14, 엑스코(대구))
권호 18권 2호
발표분야 생물공학-포스터
제목 Construction and validation of a single domain antibody (SdAb) synthetic library
초록  Due to the unique properties such as small size and high stability, single domain antibodies (SdAb: also called nanobody) offer new opportunities for a variety of diagnostic and therapeutic applications. Conventionally, single domain antibodies have been isolated by screening of heavy chains of antigen immunized camelids or cartilaginous fish. To obviate the animal immunization with a target antigen, which presents animal welfare concerns and requires more time-consuming steps, we have constructed a single domain antibody synthetic library which has diversified CDR (Complementarity Determining Region) sequences (size: > 109). For efficient screening of high affinity binders, the randomized synthetic single domain antibody nucleotides were constructed into a vector for phage display or bacterial display. For the validation of the sdAb synthetic library, high affinity single domain antibodies were screened against two model antigens, amyloid-β and Interleukin-1β and the results will be presented.
저자 주만석, 정상택
소속 국민대
키워드 Single doamin antibody; synthetic antibody library; phage display; bacterial display; antibody engineering
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