화학공학소재연구정보센터
학회 한국화학공학회
학술대회 2015년 가을 (10/21 ~ 10/23, 일산 KINTEX)
권호 21권 2호, p.1783
발표분야 생물화공
제목 L-DOPA synthesis using electrochemically pretreated graphite/nafion modified GCE
초록 Levodopa or L-3,4-dihydroxyphenylalanine (L-DOPA) is the precursor of the neurotransmitter dopamine. L-DOPA is a famous treatment for Parkinson’s disease symptoms. In this study, electroenzymatic synthesis of L-DOPA was performed in a three electrode cell, comprising a Ag/AgCl reference electrode, a platinum wire auxiliary electrode, and a glassy carbon working electrode. We conducted some immobilization modified working electrode using graphite to improve reusability of enzyme. Electrochemically activated graphite/nafion composite was prepared by using a simple electrochemical method. Optimizations of experimental variables, such as the pH, temperature, operational and storage stability experiments were investigated. Kinetic parameter of enzyme and electrode was also studied.
저자 박돈희1, Jae-Hee Park2, Seok-Hwan Park1, Hyoung-Min Lee3, Siti Fauziyah Rahman1
소속 1Interdisciplinary Program of Graduate School for Bioenergy and Biomaterials, 2Chonnam National Univ. / Department of Biotechnology & Bioengineering, 3Chonnam National Univ.
키워드 Graphite; L-DOPA; Electrochemical pretreatment; tyrosinase enzyme
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