| 초록 |
The capability to downregulate target gene expression has led to the rapid development of siRNA-based therapeutics. However, there is a major hurdle for the full exploitation in packaging and delivering multiple siRNAs to the target cells. Here, we designed self-assembled pre-siRNA cargo by complementary rolling circle transcription (cRCT). The product has repeated pre-siRNA region with spacer regions in between to provide a low steric hindrance for Dicer. This facilitates better pre-siRNA cleaving efficiency compared to the previously reported RNAi microsponge. Furthermore, the sizes of structures can be rationally controlled from the micron to sub-micron ranges by changing the ratio of circular DNA to RNA polymerase. Taken together, we envision that this pre-siRNA cargo would maximize the potential of RNAi therapeutics. |
