| 학회 | 한국고분자학회 |
| 학술대회 | 2004년 가을 (10/08 ~ 10/09, 경북대학교) |
| 권호 | 29권 2호, p.385 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | Neurogenesis of Stem Cell Transfected using PEI |
| 초록 | Viral vector to delivery gene may induce undesirable safety problem, while non-viral vector such as cationic lipids and polymers are receiving the growing attention due to safety, easy handling and preparation or inexpensive cost. PEI with cationic charge can complexe with anionic phosphate of DNA through electrostatic interaction, resulting in a reduced size of the DNA and an enhanced stability toward DNase. Efficient delivery of foreign DNA into cells is an extremely critical step in gene therapy. Meanwhile, the tracing of neurogenesis of stem cells is important to identify the properties of stem cell. Therefore, in this work, we examined the neurogenesis of stem cell after transfection using PEI. Transfection efficiency of rBMSC was about 10% in the measurement of flow cytometry and increased as N/P charge ratio increased. However, cell viability decreased with increasing N/P charge ratio. Successively, transfected rBMSC was differentiated to neural cell in the presence of neurogenesis media. We confirmed neural cell by NSE staining. In conclusion, we confirmed that PEI proved to be an efficient gene delivery carriers for stem cell and in addition, could served as tracer for neurogenesis after transfection. Acknowledgement : This work was supported by Korea Ministry of Commerce, Industry and Energy (N11-A08-1402-05-1-3). |
| 저자 | 김선화1, 김선경1, 김문석2, 조선행2, 강길선1, 이해방2 |
| 소속 | 1전북대, 2한국화학(연) |
| 키워드 | gene delivery; PEI; stem cell |
