| 학회 |
한국화학공학회 |
| 학술대회 |
2020년 가을 (10/14 ~ 10/16, e-컨퍼런스) |
| 권호 |
26권 1호, p.298 |
| 발표분야 |
생물화공 |
| 제목 |
Microbial production of 4-amino-1-butanol |
| 초록 |
4-Amino-1-butanol (4AB) is an intermediate compound for drugs and a precursor of biodegradable polymers used for gene delivery. 4AB is produced by chemical synthesis from petroleum resources and there has been no report on its production from renewable resources. Here, we report for the first time the fermentative production of 4AB from glucose by metabolically engineered Corynebacterium glutamicum. A newly designed pathway comprising a putrescine aminotransferase (encoded by ygjG) and an aldehyde dehydrogenase (encoded by yqhD) from Escherichia coli was introduced to a putrescine producing C. glutamicum strain developed in this study to produce 4AB. Next, metabolic engineering strategies such as fine-tuning the ygjG and yqhD expression levels, eliminating competing pathways, and optimizing culture condition were applied. In fed-batch culture, the final engineered strain produced 24.7 g/L of 4AB. This work was supported by the Technology Development Program to Solve Climate Changes (Systems Metabolic Engineering for Biorefineries) from the Ministry of Science and ICT through the National Research Foundation (NRF) of Korea (NRF-2012M1A2A2026556 and NRF-2012M1A2A2026557). |
| 저자 |
Prabowo Cindy Pricilia Surya1, Jae Ho Shin2, Jae Sung Cho3, Tong Un Chae1, 이상엽2
|
| 소속 |
1Metabolic and Biomolecular Engineering National Research Laboratory, 2Department of Chemical and Biomolecular Engineering, 3KAIST |
| 키워드 |
생물공정 |
| E-Mail |
|
| 원문파일 |
초록 보기 |
