| 학회 |
한국화학공학회 |
| 학술대회 |
2020년 가을 (10/14 ~ 10/16, e-컨퍼런스) |
| 권호 |
26권 1호, p.326 |
| 발표분야 |
생물화공 |
| 제목 |
CRISPR-based genome engineering of Corynebacterium glutamicum |
| 초록 |
Corynebacterium glutamicum is an important industrial bacterial host for the production of amino acids. A new genome engineering tool is needed to accelerate the development of industrially capable strains. In this study, we reported a CRISPR/Cas9-coupled recombineering system as a strategy to knock out one or more genes in C. glutamicum. First, synthetic single stranded oligodeoxyribonucleotides targeting the desired genomic region are introduced by the recombinase (RecT). The CRISPR/Cas9 complex is then used to counter-select the negative mutants. The efficient curing system was designed to perform multiple gene engineering and to obtain plasmid-free strains. Using this system, metabolically engineered C. glutamicum strain was developed for γ-aminobutyric acid production. This work was supported by the Technology Development Program to Solve Climate Changes on Systems Metabolic Engineering for Biorefineries from the Ministry of Science, ICT and Future Planning (MSIP) through the National Research Foundation of Korea [NRF-2012M1A2A2026556 and NRF-2012M1A2A2026557. |
| 저자 |
Prabowo Cindy Pricilia Surya1, Jae Sung Cho2, Kyeong Rok Choi3, Jae Ho Shin1, Dongsoo Yang2, 이상엽3
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| 소속 |
1Metabolic and Biomolecular Engineering National Research Laboratory, 2Department of Chemical and Biomolecular Engineering, 3KAIST |
| 키워드 |
생물공정 |
| E-Mail |
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| 원문파일 |
초록 보기 |
