| 학회 | 한국고분자학회 |
| 학술대회 | 2002년 가을 (10/11 ~ 10/12, 군산대학교) |
| 권호 | 27권 2호, p.315 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | Effect of Bone Induction for Ipriflavone Loading PLGA Scaffold |
| 초록 | Ipriflavone (IP), a non-hormonal isoflavone derivative, has been shown to interfere with bone remodeling by inhibiting bone resorption and stimulating bone formation. IP consistently increased the amount of 45Ca incorporated into the cell layer by MSCs. In this study, we developed the novel IP loading PLGA (IP/PLGA) scaffolds for the possibility of the application of the tissue engineered bone. IP/PLGA scaffolds were prepared by solvent casting/salt leaching method. IP/PLGA scaffolds were characterized by porosimeter, SEM, determination of residual salt amount, DSC, and X-ray diffraction. IP/PLGA scaffold were implanted into the back of athymic nude mouse, to observe the effect of IP on the osteoinduction compared with control PLGA scaffolds. Thin sections were cut from paraffin embedded tissue and histological sections were stained H&E, von Kossa, and immunohistochemical staining for typeⅠcollagen and osteocalcin. IP/PLGA of 50% were implanted on the back of athymic nude mouse to observe the effect of IP on the induction of cells proliferation for 9 weeks. The evidence of calcification, osteoblast, and osteoid from the undifferentiated stem cells in the subcutaneous sites and other soft connective tissue sites having a preponderance of stem cells has been observed. In summary, it seems that IP plays an important role for bone induction in IP/PLGA scaffolds. Acknowledgement: This study was supported by MOWH(IMT-2000, 01-PJ11-PG9-01NT00-0011) |
| 저자 | 김인영1, 장지욱2, 이상진*3, 강길선**4, 이봉5, 이영무*6, 이일우***, 한창환****, 이해방***** |
| 소속 | 1부경대, 2*한양대, 3**전북대, 4*** 가톨릭의과대, 5**** 가톨릭의과대, 6*****한국화학(연) |
| 키워드 | |
