| 학회 |
한국공업화학회 |
| 학술대회 |
2019년 가을 (10/30 ~ 11/01, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 |
23권 2호 |
| 발표분야 |
(생물공학) 생물공학의 미래기술 |
| 제목 |
Broad-spectrum, fine-tuning gene expression using synthetic sRNA in Escherichia coli |
| 초록 |
Gene expression regulation is critical for constructing cell factories and genetic circuits to balance and control system-wide fluxes. sRNAs are short non-coding RNAs that can control the expression of target genes in trans at the translational level in prokaryotes. In this talk, I introduce a genome-wide gene expression control system based on synthetic sRNAs, which utilizes the abilities and characteristics of natural sRNAs in Escherichia coli. Plasmid-based expression of sRNAs enables target gene expression control without modification of chromosome, thus allowing high-throughput screening of targets for metabolic engineering. Synthetic sRNAs can finely regulate gene expression by modulating an mRNA-binding chance and sRNA abundance. The engineered sRNA scaffold provides a wider range of gene repression, and even represses expression to levels beyond the intrinsic ability of the original sRNA. This will be useful for fundamental and applied biological and biotechnological studies. |
| 저자 |
유승민 |
| 소속 |
중앙대 |
| 키워드 |
synthetic sRNA; gene expression; scaffold; cell factory; genetic circuit
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| E-Mail |
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