| 학회 |
한국공업화학회 |
| 학술대회 |
2019년 가을 (10/30 ~ 11/01, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 |
23권 2호 |
| 발표분야 |
포스터_생물공학 |
| 제목 |
Development of RNA-based caprolactam sensor for high-throughput screening and selection |
| 초록 |
The Microbial strain which produces caprolactam, a monomer widely used for the synthesis of nylon-6, has been recently reported. One of the hurdles for screening high-producer is that the recombinant strain coproduce caprolactam and valerolactam which differs by only a single carbon. Therefore, it is pivotal for high-throughput screening to specifically report intracellular concentration of caprolactam. However, a highly specific caprolactam sensor has not been reported. Here, we developed an artificial riboswitch that specifically responds to the caprolactam using a coupled in vitro−in vivo selection strategy. Heterogeneous pool of RNA aptamers obtained from SELEX is inserted between ORF of reporting gene in a plasmid to proceed in vivo selection. Constructed caprolactam sensor was activated by 3.36-fold in the presence of 50 mM caprolactam and enabled caprolactam-dependent control of cell growth, which will be useful for improving bio-based manufacture and metabolic engineering.
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| 저자 |
김민선1, 장성연1, 장성호1, 임대균2, 강택진3, 오민규2, 정규열1
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| 소속 |
1포항공과대, 2고려대, 3동국대 |
| 키워드 |
Caprolactam; Aptamer; Riboswitch; SELEX; high-throughput
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| E-Mail |
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