| 학회 |
한국고분자학회 |
| 학술대회 |
2012년 봄 (04/12 ~ 04/13, 대전컨벤션센터) |
| 권호 |
37권 1호 |
| 발표분야 |
고분자가공/복합재료 |
| 제목 |
Osteogenic Differentiation of Human Mesenchymal Stem Cells on the Electrospun Poly-L-lactide Composite Nanofibers Incorporated with Calcium phosphate prepared by Electron Beam Irradiation |
| 초록 |
As an alternative strategy to induce bone regeneration, various calcium phosphates have been prepared by acid-base reaction, precipitation, and microwave methods, which have successfully regulated osteogenic differentiation of the osteoblasts and human mesenchymal stem cells (hMSC) after incorporating with proper scaffolds. In this study, we synthesized calcium phosphate (CaP) with electron beam and then incorporated it with electrospun PLLA nanofibers. We obtained biphasic calcium phosphate (BCP) and hydroxyapatite (HAp) by controlling the pH under electron beam irradiation, and confirmed its properties through SEM, XRD, and FT-IR. The biocompatibility of the nanofibers was evaluated using hMSC, and the adherent cell morphology and cell viability of each sample were examined using fluorescence straining and a cell count kit-8 at different time intervals. Moreover, for the potential osteogenic market, alkaline phosphatase (ALP) activity was measured after 7 days of differentiation. |
| 저자 |
조선영, 신영민, 김용수, 박종석, 권희정, 노영창, 임윤묵
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| 소속 |
한국원자력(연) |
| 키워드 |
Calcium phosphate; Poly-L-lactide; Electrospinning; Osteogenic differentiation
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| E-Mail |
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