| 초록 |
Alginate, an acidic linear polysaccharide, is composed of (1-4)-linked β-D-mannuronate (M) and its C5-epimer α-L-guluronate (G). Recently, brown seaweed containing alginate as the major carbohydrate has been considered as renewable biomass for the production of biofuels and biochemicals. In this study, we investigated alginate saccharification using the recombinant oligoalginate lyase (OAL) of Stenotrophomonas maltophilia KJ-2 to obtain alginate monosaccharide, unsaturated uronate. Saccharification efficiency greater than 80 % was obtained by batch saccharification of 1 %(w/v) alginate using 400 μg/ml of the recombinant Escherichia coli cell lysates containing KJ-2 OAL at pH 7.5 and 40 oC. Under 4 %(w/v) alginate, the saccharification with efficiency greater than 50 % was obtained using 1200 μg/ml of the recombinant Escherichia coli cell lysates containing KJ-2 OAL at pH 7.5 and 40 oC. |
