| 초록 |
We report the first functional surface display of Bacillus megaterium tyrosinaseon Bacillus subtilis spores using CotE as an anchor protein. Flow Cytometry was used to verify surface expression of tyrosinase on the purified spores. Moreover, tyrosinase activity of the displayed enzyme on B. subtilis spores was monitored in the presence of L-tyrosine (substrate) and CuSO4 (inducer). In addition, the tyrosinase-expressing spores could be repeatedly used with 62% retained enzymatic activity after six times washing with Tris-HCl buffer. Different concentration of tyrosine was detected from 0.0001 nM to 1 mM in urine after formation of color spectrums from bright brownish-gray to dark brownish-gray during 1 hour. Study on the patient groups also showed that the kit can screen three groups of patients (diabetics, diabetics/ chronic kidney disease, chronic kidney disease) from the healthy persons. |
