| 초록 |
Poly(ethylene glycol) (PEG) has been prominently used as the basis of solid supports due to its hydrophilic properties. However, limitation regarding low purity of peptide synthesis still exists. To overcome the drawback, we designed polymer supports generated from microfluidic channels. The resins were prepared by dissolving various monomers and their appropriate crosslinker at varying ratios. The physicochemical properties of each resin were compared with that of the conventionally used TentaGel S NH2. Such characteristics were evaluated by assessing the: 1) swelling properties, 2) cell-adhesive behavior of RGD tripeptide-bound resins and 3) purity of a complicated Jung-Redemann decapeptide. Among these resins, PEG-based resins with a higher degree of cross-linker (PEGA:PEGDA of 3:5 ratio) presented favorable results. Hence, we anticipate that such polymer support to be used as a cost-efficient alternative for conventional resins as well as in the application of on-bead bio-assays. |
