| 학회 | 한국공업화학회 |
| 학술대회 | 2017년 봄 (05/10 ~ 05/12, 광주 김대중컨벤센센터(Kimdaejung Convention Center)) |
| 권호 | 21권 1호 |
| 발표분야 | 생물공학 |
| 제목 | Novel sensitive MDR/XDR Mycobacterium tuberculosis detection method using ligation-based amplification coupled with high resolution CE-SSCP |
| 초록 | Multi-drug resistant Mycobacterium tuberculosis (MDR-TB) is causing serious problems for public health. So rapid detection of MDR-TB is really important. In addition, because its mechanism is caused by gene mutation, efficient diagnosis for MDR-TB requires gene-based molecular diagnosis method. Conventional gene-based molecular diagnosis technologies are likely to cause false-negative or false-positive problems due to non-specific hybridization among used templates, probes, and primers. Also, they can detect only limited number of genes. In this study, we report gap filling ligation and ligase detection reaction coupled with high-resolution CE-SSCP (MGLAD-CE-SSCP) to amplify and detect MDR-TB. By using this, false-positive and negative results from non-specific hybridization can be minimized. Moreover, this method can rapidly and simultaneously detect MDR-TB bacteria. |
| 저자 | 전성민, 최웅, 신기영, 정규열 |
| 소속 | 포항공과대 |
| 키워드 | MDR-TB; gap filling ligation; ligase detection reaction; CE-SSCP |
