| 학회 |
한국고분자학회 |
| 학술대회 |
2003년 가을 (10/10 ~ 10/11, 부경대학교) |
| 권호 |
28권 2호, p.172 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
Tissue-Engineered Vascular by in vivo Culturing of Smooth Muscle Cells in Poly(L-lactide-co-ε-caprolactone) Scaffolds |
| 초록 |
Vascular smooth muscle cells (VSMCs) were isolated from rabbit aortas, seeded in biodegradablr porous polymer scaffolds, and implanted subcutaneously in the dorsum of sthymic nude mice to tissue engineer vascular smooth muscle tissue in vivo. Poly(L-lactide-co-ε-caprolactone)(PLCL) copolymer showed a rubber-like elastic characteristic, in addition to an amorphous property and fast biodegradability. SMCs were seeded in 1×107 cells/ml densities.Before implantation, the scaffold-cell constructs were cultured in vitro for 5hour. The implants were harvested after in vivo incubation of 2, 5, 8, and 16weeks. The implants were examined SEM, histochemically by haematoxylin and eosin staining, by Masson's trichrome staining, and immunohistolochemically by SM -actin, myosin heavy chain, and CM-DiI labeling. The percent of smooth muscle extracellular matrices increased, while neovascularization decreased with an increased implantation period. The morphology of smooth muscle tissue, the expression of smooth muscle matrices in the PLCL scaffold, was similar to those in native vascular. PLCL scaffold facilitated cell seeding and promoted the in vivo formation of vascular smooth muscle tissue.
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| 저자 |
정성인1, 김수현1, 김병수2, 김동익3, 권재현1, 강선웅2, 조승우4, 김영하1
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| 소속 |
1한국과학기술(연), 2한양대, 3성균관대, 4서울대 |
| 키워드 |
Elastic polymer; Smooth muscle cells; Biodegradable polymer; Tissue engineering
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| E-Mail |
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