| 학회 |
한국고분자학회 |
| 학술대회 |
2009년 봄 (04/09 ~ 04/10, 대전컨벤션센터) |
| 권호 |
34권 1호 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
Chitosan nanoparticles functionalized by M cell targeted peptide |
| 초록 |
Identification of the markers recognized by M cells is vital to produce effective oral vaccines targeting M cell in the Peyer`s patch (PP). By utilizing phage display together with an M cell model, we isolated a specific M cell-targeting phage clone, encoding CKSTHPLSC (CKS9) and validated the specificity of this peptide to the PP ex-vivo. The CKS9-immobilized chitosan nanoparticles (CKS9-CNs) were prepared by ionic gelation method. The specific binding of the CKS9-CNs to the M cell model in vitro was visualized by confocal laser scanning microscopy and their targeting ability to the M cells in the PP of the intestine in vivo was also investigated through the closed-ideal loop assay and optical images of mice. In vitro and in vivo results indicated that the CKS9-CNs possess the more specific accumulation property into M cell in the PP in comparison with control. Therefore, this study opens the possibility to provide a new carrier for oral vaccines delivery to the small intestine. |
| 저자 |
유미경1, 최진혁1, 강상기1, 전희선1, 최윤재1, 박인규2, 나희삼2, 이현철2, 조종수1
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| 소속 |
1서울대, 2전남대 |
| 키워드 |
M cells; targeting; phage display; chitosan nanoparticles
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| E-Mail |
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