| 초록 |
Photodynamic therapy (PDT) is an attractive strategy for cancer treatment. However, the hydrophobicity of PS limits the clinical applications of PDT, because that may cause the suboptimal half-life, lack of specificity and skin photosensitivity. In this study, we designed and synthesized a multifunctional system, heparin-folate-photosensitiser conjugates (HFP), for cancer treatment based on PDT. The folate ligands and pheophoebide a (Pheo) were conjugated to heparin backbone through the chemical modification. The chemical structures of HFP were characterized by 1H NMR. The coupling ratio of Pheo can be easily controlled by the feed molar ratio of Pheo to heparin, which was studied by UV spectrometer. The HFP can form self-assembled nanoparticles in aqueous medium with the diameters about 150 nm. In the in vitro cell studies, the HFP nanoparticles showed good cellular uptake and marked photocytotoxicity after light treatment, causing significant damage to cancer cell. |
