| 초록 |
Polyethylene terephthalate (PET) waste has caused serious environmental pollution. Recently, PET depolymerization by enzymes with PET-depolymerizing activity has received attention as a solution to recycle PET. An engineered variant of leaf branch compost cutinase (293 amino acid), ICCG, showed excellent depolymerizing activity toward PET at 72 °C, which was the highest depolymerizing activity and thermo-stability ever reported in previous works. To simplify the purifcation steps of ICCG, we developed a secretory production system using Bacillus subtilis and its 174 types of N-terminal signal peptides. In this study, we developed a secretory production system of the engineered cutinase with PET-depolymerizing activity to obtain high amounts of the enzyme by a relatively simple purification method. This system will contribute to the recycling of PET waste via a more efficient and environmentally friendly method based on enzymes with PET-depolymerizing activity |
