| 학회 | 한국공업화학회 |
| 학술대회 | 2022년 봄 (05/11 ~ 05/13, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 | 26권 1호 |
| 발표분야 | 포스터-생물공학 |
| 제목 | Enhancement of CO2 tolerance by expressing a single proton pump enables microalgal valorization of industrial flue gas |
| 초록 | Microalgae can accumulate various valuable products, but theirreal-world applications are hindered by their CO2 susceptibility.Herein, the transcriptomic changes in a model microalga, Chlamydomonasreinhardtii, in a high-CO2 milieu (20%) are evaluated. Theprimary toxicity mechanism consists of aberrantly low expression of plasmamembrane H+-ATPases (PMAs) accompanied by intracellularacidification. Our results demonstrate that the expression of a universallyexpressible PMA in wild-type strains makes them capable of not only thriving inacidity levels that they usually cannot survive but also exhibiting 3.2-foldincreased photoautotrophic production against high CO2 viamaintenance of a higher cytoplasmic pH. A proof-of-concept experiment involvingcultivation with toxic flue gas shows that the production of CO2-basedbioproducts by the strain is doubled compared with that by the wild-type,implying that this strategy potentially enables the microalgal valorization ofCO2. |
| 저자 | 양하은1, 최홍일2, 심상준1 |
| 소속 | 1고려대, 2한국생명공학(연) |
| 키워드 | Microalgae; CO2 tolerance; industrial flue gas; H+ pump |
