| 학회 | 한국공업화학회 |
| 학술대회 | 2022년 봄 (05/11 ~ 05/13, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 | 26권 1호 |
| 발표분야 | 포스터-생물공학 |
| 제목 | Extracellular vesicle protein based fluorescent gold nanolcusters for cellular imagingapplication |
| 초록 | Recently, as the field of moleculardiagnostics grows, bioimaging and biosensing technologies based on fluorescentmetal nanocluster are being actively studied. Especially protein-basedfluorescence gold nanoclusters (AuNCs) are attracting attention due to theirhigh fluorescence signal and biocompatibility. We successfully synthesizedAuNCs based on extracellular vesicle (EVs) proteins. Specifically, we developedtwo different EVs based AuNCs (EV-AuNCs) with independent blue and redfluorescence emission using neutral (pH 7) and alkaline (pH 11.5) pH. Wevalidated the successful synthesis of EVAuNCs by FESEM, EDX, NTA and XPS. BothEVAuNCs were incubated with MCF, HeLa and HT29 cells, red emitting EVAuNCs werenot toxic, whereas the blue emitting EVAuNCs were toxic at high concentrations(≥5mg/ml). Additionally, we succeeded instaining cancer cell nucleus using red emitting EVAuNCs. EVAuNCs is the firststudy to imaging cells by synthesizing fluorescence nanomaterials in EVs. |
| 저자 | 이은성, 박기수 |
| 소속 | 건국대 |
| 키워드 | Extracellular vesicles; gold nanocluster; Fluorescence; bioimaging |
