| 학회 | 한국고분자학회 |
| 학술대회 | 2003년 봄 (04/11 ~ 04/12, 연세대학교) |
| 권호 | 28권 1호, p.253 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | Evaluation of Tissue Engineered Spinal Cord using Bone Marrow-derived Stromal Cells Seeded Polymer Scaffolds |
| 초록 | Bone marrow-derived stromal cells (BMSCs) were harvested from the femur of adult female Fischer rats. BMSCs were suspended at 2 × 107 cells/mL in a 25% Pluronic F127 solution and then polyglycolide (PGA) nonwoven was saturated with 50 L Pluronic F127 solution. Fischer rats received an implant consisting of the BMSCs suspended in the Pluronic F127/PGA nonwoven in the gap (T8 ~ T9; 5 mm) created by the spinal cord resection. For histological evaluation, the implants were removed after 4 and 8 weeks. Thin sections were cut from paraffin embedded tissue and histological sections were stained hematoxylin and eosin (H&E) staining and immunohistochemical staining for neurofilament (NF) and S-100 protein. It was observed that the effect of Pluronic F127/PGA nonwoven with BMSCs on neuroinduction are stronger than Pluronic F127/PGA nonwoven without BMSCs and blank (negative control). In conclusion, it seems that BMSCs plays an important role for spinal cord regeneration of tissue engineering area. Acknowledgement: This work was supported by Korea Ministry of Commerce, Industry and Energy (N11-A08-1402-05-1-3). |
| 저자 | 최진산;이상진;박기숙;장지욱;이봉;이영무;이일우;이혜경;강길선;이해방 |
| 소속 | 전북대 |
| 키워드 | tissue engineering; spinal cord regeneration; bone marrow-derived steomal cell |
