| 학회 |
한국화학공학회 |
| 학술대회 |
2003년 가을 (10/24 ~ 10/25, 한양대학교) |
| 권호 |
9권 2호, p.2163 |
| 발표분야 |
생물화공 |
| 제목 |
Comparative Proteome Analysis of Hansenula polymorpha DL1 and A16 |
| 초록 |
Proteomic responses of methylotrophic yeasts (Hansenula polymorpha DL1 and A16) to growth medium tuning by carbon source shift were monitored and analyzed through two-dimensional gel electrophoresis. Through comparative proteome analyses, the intracellular proteins showing complex expression patterns were systematically sorted into: 1) proteins that are commonly expressed with comparable high abundance in both strains; 2) strain-specific proteins that are expressed at high level only in a particular strain; 3) strain-specific and methanol-induced proteins that are expressed only in the presence of methanol; and 4) strain-specific and constitutively-expressed proteins that are expressed consistently irrespective of carbon source shift without extreme change in expression level. As a result, DL1 has such distinct metabolic characteristics as enhanced metabolic activities both in glycerol uptake and glyoxylate by-pass cycle, as compared to A16. Our systematic approach to the detailed analysis of proteomic responses will be greatly useful in more completely understanding global physiologies of H. polymorpha, as sufficient proteome databases for various methylotrophic yeasts are established further. |
| 저자 |
김양훈1, 한경연1, 이기범2, 강현아2, 이상기2, 이지원1
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| 소속 |
1고려대, 2한국생명공학(연) |
| 키워드 |
<i>Hansenula polymorpha</i>; proteomic responses; comparative analysis of 2-D PAGE gels; metabolic characteristics |
| E-Mail |
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| 원문파일 |
초록 보기 |
