| 초록 |
In this study, the effects of various buffers and ionic strengths on the immobilization of Candida rugosa and Rhizops oryzae lipases were investigated to enhance the activities of the immobilized lipases. Among the various buffers, the optimal buffers and ionic strength for the immobilization and co-immobilization of C. rugosa and R. oryzae lipases were determined to be a mixture of 0.25 M MOPs and sodium phosphate buffer (pH 6.5). Moreover, the activities of immobilized and co-immobilized C. rugosa and R. oryzae lipases under their optimal conditions were 3756.11, 2845.21 and 16505.6 U/g matrix, respectively.Finally, the activities of the immobilized lipases were maintained at levels greater than 90 % of their original activities after ten reuses and at levels greater than 60 % of their original activities after twenty reuses. Moreover, the activities of the co-immobilized lipases were maintained at levels exceeding 90 % and 70 % of their original activities after thirteen reuses and twenty reuses, respectively. |
