| 초록 |
Epidermal growth factor receptor (EGFR) 19 deletion mutation gene is often over-expressed in carcinoma patient. EGFR 19 deletion mutation is known as typical biomarker of non-small cell lung cancer, which one section in the coding exon 19 of EGFR is deleted. Therefore, there have been many attempts over the years to detect EGFR 19 deletion mutation gene for replacing conventional diagnostic method such as PCR and tissue biopsy. We developed an efficient and facile detection platform based on Rolling Circle Amplification (RCA), which provides high sensitivity and simple procedure including isothermal amplification. RCA is suitable for detecting EGFR 19 deletion mutation gene using liquidbiopsy that is circulating through blood system in the form of proteins, genes. Limit of detection (~50 nM) and a faster detection time (~30 min) could be achieved by introducing RCA. |
