| 초록 |
Middle East respiratory syndrome(MERS) is a viral respiratory disease caused by a coronavirus (MERS‐CoV). About 3-4 out of 10 MERS patients have died. A novel application of rolling circle amplification (RCA) as a biosensor for MERS RNA pathogen detection is introduced. Following WHO indication, a facile and robust microfluidic array chip assay has been developed based on RCA of adaptor templates upon the hybridization of MERS pathogens. Long single strands of RCA products can be visualized by florescent dye staining. When a real sample containing MERS pathogen is applied, RCA of the DNA template can be achieved and can be visualized by under a UV lamp. In contrast, if there is no MERS pathogen in sample, no fluorescence signal can be seen. This assay shows high sensitivity and potent ability to detect various disease according to design of DNA template. It is likely that the development of this facile and sensitive MERS pathogen detection assay may relieve excessive workloads. |
