화학공학소재연구정보센터
학회 한국고분자학회
학술대회 2008년 봄 (04/10 ~ 04/11, 컨벤션 뷰로(대전))
권호 33권 1호
발표분야 의료용 고분자
제목 Bio-imaging of LYVE-1 Receptor-Mediated Endocytosis of Hyaluronic Acid Derivatives Using Quantum Dots
초록 Hyaluronic acid (HA) plays important roles in the body through the interaction with receptors on cells. Among the receptors, LYmphatic Vessel Endothelial hyaluronan recepor-1 (LYVE-1) abundantly exist in the liver and the lymphatics with a high specificity to HA. We investigated the effect of chemical modification of HA on its LYVE-1 receptor-mediated endocytosis using quantum dots (QDots). B16F1 cells expressing LYVE-1 were incubated with HA-QDot and analyzed with a confocal microscope and a well plate reader. The results clearly showed a reduction of internalization via receptor-mediated endocytosis with the increasing degree of HA modification. Using near-infrared emission QDots, we confirmed that HA-QDot conjugates with a low degree of HA modification maintaining enough binding sites for HA receptors were mainly accumulated in the liver, while those with a high degree of HA modification losing much of HA characteristics were evenly distributed to the body.
저자 김기수, 김지석, 강현구, 한세광
소속 포항공과대
키워드 Bio-imaging; LYVE-1; Endocytosis; Hyaluronic Acid; Quantum Dots
E-Mail skhanb@postech.ac.kr