| 초록 |
Nanocarriers to deliver therapeutic molecules have gained great interest in the field of tissue engineering. However, delivery efficiency has often been limited to satisfy the therapeutic efficacy. Hence, we designed hollow mesoporous silica nanocarrier (HMS) to achieve a high loading capacity and to enable controlled and sustained delivery. HMS was prepared by replicating inorganic nanorods with silica phase through sol-gel reaction and acid-etching. The nanocarriers were characterized by TEM, EDX, FT-IR, and BET. As a therapeutic molecule for osteoblastic cell stimulation, we delivered CKIP1 small interference RNA (siRNA) through HMS. The cell viability, intracellular uptake, gene silencing, and bone-related gene expressions were investigated. Rod-shaped and hollow structured nanocarriers with mesoporous structure were well detected from TEM images. The hollow structure increased the surfaces area and pore volume around 22-45%; as a result, the siRNA loading efficiency of HMS was about 3-fold higher than that of the non-hollow form. Furthermore, the release of siRNA from the HMS was sustained up to 2-3 weeks. Cells were highly viable to the nanocarriers treated over a wide concentration range. The siRNA-loaded HMS was effectively delivered into cells, with the cellular uptake level of ~90%. The delivered siRNA/HMS could silence the expression of the target gene down to 80%, which in turn upregulated the expression of bone-associated genes. Taken all, the HMS can be a potential delivery system of gene, for the osteoblastic stimulation in bone regeneration field. |
