| 학회 |
한국공업화학회 |
| 학술대회 |
2019년 봄 (05/01 ~ 05/03, 부산 벡스코(BEXCO)) |
| 권호 |
23권 1호 |
| 발표분야 |
생물공학_포스터 |
| 제목 |
Pathway optimization of biosynthetic pathway of Escherichia coli through fine-tuning gene expression level based on synthetic chemical-sensing genetic devices for enhanced production of flavonoids |
| 초록 |
Naringenin is one of the high value-added compounds in that it is a key precursor for diverse flavonoids and has pharmaceutical activities by itself. Optimization of biosynthetic pathways in microbes for increased production of flavonoids is necessary, but it is difficult to efficiently optimize gene expression level. Here, fine-tuning tools to control gene expression involved in naringenin production at both transcription and translational levels were thoroughly studied. As a further step, mutant libraries based on the fine-tuned gene expression were generated, and pathway optimization was finally conducted through screening the mutant libraries based on synthetic chemical-sensing genetic devices with a high-throughput screening capability. We demonstrate that the semi-rational system based on biosensors can be an effective strategy for efficient optimization of biosynthetic pathways of flavonoids and apply for various issues involved in optimization of gene expression level. |
| 저자 |
황현규, 장성호, 정규열
|
| 소속 |
POSTECH |
| 키워드 |
Metabolic engineering; Pathway optimization; Biosensors; Naringenin; Flavonoids
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| E-Mail |
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