| 초록 |
In this study, FePd magnetic nanoparticles (NPs) were developed as artificial enzymes with high biocompatibility and reusability, named ‘magnetozymes’, in a one-pot aqueous synthesis method using glutathione (GSH) and cysteine (Cys) as surfactants. The prepared hydrophilic FePd magnetozymes can be successfully re-dispersed in water. The saturation magnetizations of the Cys- and GSH-conjugated magnetozymes, measured by a superconducting quantum interference device, are 4.7 and 41.4 emu/g at 300 K. The catalytic activities were tested by measuring the reduction of fluorescent dye and hydrogen peroxide by optical absorption measurements and electrochemical characterization. The Cys-FePd and GSH-FePd NCs exhibited significantly enhanced efficiency, with catalytic constants more than 2- and 7- fold higher than horseradish peroxidase (HRP), respectively. Furthermore, in vitro experiments reveal that the FePd NPs clearly behave like peroxidase to reduce ROS levels in mammalian cells. |
