| 학회 | 한국공업화학회 |
| 학술대회 | 2022년 봄 (05/11 ~ 05/13, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 | 26권 1호 |
| 발표분야 | 포스터-생물공학 |
| 제목 | Engineering of CYP153A33 to enhance ω-hydroxylation to overoxidation activity ratio in the biotransformation of medium-chain primary alkanols |
| 초록 | Medium-chain α,ω-alkanediols are a promising feedstock for polymer and chemical industries. Of these, α,ω-dodecanediol is a versatile chemical used as lubricants, adhesives, crosslinking agent and monomer fo polyesters, polyurethane, and polyamide. CYP153A33 from Marinobacter aquaeolei is known for its dodecane/1-dodecanol ω-specific oxidation activity. In this study, CYP153A33 was engineered to increase ω-specific oxidation activity. Based on structural understanding, several CYP153A33 variants were evaluated and P136A showed a significant increase in α,ω-dodecanediol production(7.12 mM from 10 mM 1-dodecanol) with an increased hydroxylation to over-oxidation activity ratio. Finally, the biotransformation activity of CYP153A33 P136A against medium-and long-chain primary alkanols was also evaluated. |
| 저자 | 최권영, 박현아 |
| 소속 | 아주대 |
| 키워드 | CYP153A33; 1-alkanools; omega-oxidation; whole-cell biotransformation |
