| 초록 |
We developed a systematic framework which generates metabolic pathways along with their candidate enzymes in order to identify feasible biosynthetic pathways for the target chemicals. The framework largely consists of two parts, route generation and prioritization process. Route generation process generates pathways based on reaction rule sets. After the route generation, two prioritization factors, including structural similarities of the involved chemicals and thermodynamic favorability, were used to estimate the feasibility of each generated reaction pathway from the framework. As a demonstration, acrylic acid, 3-hydroxypropionate (3HP) and 6-aminocaproic acid production pathway were predicted by this platform. [This work was supported by the Technology Development Program to Solve Climate Changes on Systems Metabolic Engineering for Biorefineries from the Ministry of Science, ICT and Future Planning (MSIP) through the National Research Foundation (NRF) of Korea (NRF-2012M1A2A2026556 and NRF-2012M1A2A2026557).] |
