| 초록 |
Dual functional nanoparticles, for example metal nanoparticle with additional fluorescence property, have attracted interest biomedical imaging because they could produce more accurate images than single functional counterpart could. Colloidal synthesis of dual functional nanoparticles has been achieved by coating thin silica layer on metal nanoparticles, followed by the attachment of fluorescent molecules. However, this method requires precise control over surface chemistry and the resulting dual functional nanoparticles generally suffer from low endocytosis efficiency. Here we propose the synthesis of liposome/metal hybrid nanoparticles with embedded fluorescent molecules for a higher endocytosis efficiency. First, fluorescent Nile Red is inserted in between two lipid layers due to hydrophobic interaction. Then, during the formation of liposome, ascorbic acid is selectively encapsulated within liposome. Finally, metal ion diffuses into the liposome, and is reduced to nanoparticles within liposome. As-made hybrid nanoparticles are characterized by TEM and UV-vis spectrometer. Their optical properties are addressed by dark-field microscopy and fluorescence microscopy. |
