| 학회 | 한국재료학회 |
| 학술대회 | 2016년 가을 (11/16 ~ 11/18, 경주 현대호텔) |
| 권호 | 22권 2호 |
| 발표분야 | B. 나노화학/바이오 재료 분과 |
| 제목 | Construction of Linear Green Fluorescent Protein Oligomers as Monodisperse Supramolecular Assemblies with Defined Valency |
| 초록 | Sophisticated self-assembly structures formed by biomolecules offer new materials with high biocompatibility, biodegradability and innate stability in water.[1] These properties make bio-assemblies highly attractive as new biocatalysts, bio-devices, synthetic vaccines, cellular observation tools and for drug delivery systems. Especially, using proteins as building blocks for supramolecular polymers has come into the spotlight by virtue of the abundance of functionality from the variable amino acids. To assemble and control supramolecular architectures, a specific interaction pair(or pairs) that assembles into a well-organized cluster is needed. Selecting proper protein or peptide interaction pairs and designing assembling strategies are the keys in the field of supramolecular protein assemblies. Here we developed a co-expression system of two proteins that are self-assembled in E.coli to produce linear forms of supramolecular GFP polymers. Linear GFP polymers can be purified with discrete sizes. These oligomers are fully genetically encodable (so fusible to other proteins and produced in cells), suitable for visualization (with its fluorescent nature), and can be isolated in a discrete size. This present linear GFP polymer is highly flexible having a higher degree of freedom in its shape and range than polygon form[2]; it would broaden the range of available applications and observable events in biological processes. References [1] Bastings et al., Macrocyclization of enzyme-based supramolecular polymers, Chem. Sci., 2010, 1, 39, 3351-3357 [2] Kim et al., Green fluorescent protein nanopolygons as monodisperse supramolecular assemblies of functional proteins with defined valency, Nat. Commun., 2015, 6, 7134 |
| 저자 | 김유나, 정용원 |
| 소속 | KAIST |
| 키워드 | protein assembly; supramolecular biopolymer |
