| 초록 |
Paper-based biosensors are widely used in many areas such as point-of-care test because they are affordable and portable. For reproducible quantitative analysis, it is important to fix biomolecules to the biosensor. Conventional techniques rely on physical adsorption, which can be easily removed by physical forces. Thus, it is difficult to obtain reproducibility as a result of the analysis of biosensor using physical adsorption. To overcome the limitation, this study present a oxidation method of cellulose to covalently bind protein on paper. This method consists of three processes: oxidation of paper, the formation of Schiff base, and reduction of paper. FT-IR analysis was used to qualitatively analyze the formed aldehyde groups and imine bonds. Thus, we demonstrate a simple method to immobilize biomolecules such as enzymes and antibodies. We expect this immobilization method to increase the reproducibility of quantitative detection for commercialization of paper-based sensors. |
