화학공학소재연구정보센터
학회 한국화학공학회
학술대회 2022년 봄 (04/20 ~ 04/23, 제주국제컨벤션센터)
권호 28권 1호, p.1033
발표분야 [주제 12] 화학공학일반(부문위원회 발표)
제목 Water-wettable Void Plasmonic Nanogap for Rapid Amplification and Optical Detection of DNA
초록 Polymerase chain reaction (PCR) is considered as a golden standard in molecular diagnostics for a multitude of applications ranging from precision medicine to point-of-care testing. However, adequate amplification of DNA for conventional PCR requires several hours due to large reaction volumes of several tens of microliters. Therefore, a novel PCR technique for the rapid amplification is highly desirable for early diagnosis of infectious diseases such as COVID-19. Here, we propose water-wettable void plasmonic nanogap between metal nanoparticle and film as a novel platform for the rapid amplification and optical detection of DNA. The void nanogap is directly visualized by electron microscope. We successfully amplified DNA within few minutes based on photothermal conversion in the nanogap. Then, the amplified DNA is sensitively detected in situ via surface-enhanced Raman spectroscopy without labeling.
저자 황금래, 조유승, 강태욱
소속 서강대
키워드 재료(Materials)
E-Mail twkang@sogang.ac.kr
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