| 초록 |
Fe3O4 core particles with a size of 9 nm were prepared by co-precipitation of Fe3+, Fe2+ and NH4OH, and then silica coated on the surface of the Fe3O4 nanoparticles by the hydrolysis of TEOS. The thickness of coated silica particles can be controlled by changing the experimental parameters. 3-APTES was covalently coupled to the surface of the magnetic silica nanoparticles. Bovine serum albumin (BSA) was covalently immobilization onto the amino-silane modified magnetic silica supports by the glutaraldehyde method. The mopology, particle size, and magnetic properties of silica-coated Fe3O4 nanoparticles were characterized bt TEM, DLS and VSM. As a results, silica-coated Fe3O4 nanoparticles have an average particle size of 15 nm were obtained and superparamagnetic properties of the nanoparticles were found out. In addition, the analysis of X-ray diffraction measurements showed that the structure of the nanoparticles were spinel type. The results shows that such amino-silane modified magnetic silica is a effective superparamagnetic supports for bioseparation and the maximum BSA immobilization capacity (up to 35mg/g) was obtained 0.1M phosphate buffer at pH 5.0. |
