| 학회 |
한국화학공학회 |
| 학술대회 |
2013년 봄 (04/24 ~ 04/26, 광주 김대중컨벤션센터) |
| 권호 |
19권 1호, p.355 |
| 발표분야 |
생물화공 |
| 제목 |
Protein nanoparticle probe-polyacrylamide copolymer hydrogel based diagnostic assay platform |
| 초록 |
Protein nanoparticle probe(PNP)-polyacrylamide(PAA) copolymer hydrogel was synthesized through a simple one-step copolymerization and used as a diagnostic assay platform to break through traditional problems (i.e. low sensitivity and specificity, infeasibility of multiplex assay, random orientation of probes, probe instability, uncontrollable probe loading, etc.) of ELISA based assays. Here we show superior advantages of the diagnostic assays based on PNP-PAA copolymer hydrogel: probe immobilization without random orientation problem, controllable loading capacity of homogeneously oriented probes, protein-friendly environment, sufficient storage stability, much higher sensitivity than ELISA, and high specificity and reproducibility even in multiplex assays. PNP-PAA copolymer hydrogel was successfully applied to the sensitive and specific diagnostic assays of acquired immune deficiency syndrome (AIDS) and Sjögren’s syndrome (SS). |
| 저자 |
이은정, 이종환, 김현진, 박동현, 강윤식, 이보람, 이지원
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| 소속 |
고려대 |
| 키워드 |
Hydrogel; Protein nanoparticle; bioassay
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| E-Mail |
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| 원문파일 |
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