| 초록 |
Numerous bacteria have flagella that make them capable of swimming through water-based environments. The bacterial flagella are driven by a reversible rotary nano-machine made up of protein. The engine is powered by proton- or the sodium-motive force. A method to measure bacteria motion should be a useful tool for various questions of bacterial movement. However, the detection of bacterial motion is difficult because bacteria are too small that they are bounced around randomly by water molecules. Here in, we showed a new technique for measuring the motion of a single bacterial flagellum by Atomic Force Microscope (AFM) with a modified cantilever. The reconstruction of AFM probe was carried out by Focused Ion Beam (FIB) procedure and hydrogel coating of the cantilever. Bacterial motions were characterised by atomic force microscope, nanoindentation mode and scanning electron microscope. |
