| 학회 |
한국재료학회 |
| 학술대회 |
2016년 가을 (11/16 ~ 11/18, 경주 현대호텔) |
| 권호 |
22권 2호 |
| 발표분야 |
B. 나노화학/바이오 재료 분과 |
| 제목 |
Fabrication of porous BCP granule scaffold loaded with hyaluronic acid-gelatin hydrogel for bone regeneration |
| 초록 |
To replace and regenerate lost bone, Biphasic calcium phosphate (BCP) based synthetic bone substitutes are traditionally used today. An ideal bone graft or scaffold should be made of biomaterials that imitate the structure and properties of natural bone ECM. Porous BCP granule scaffold has a good biocompatibility and mechanical strength but it is difficult to handle for each scaffold to implant defect site. For consideration this, we incorporated HyA-Gel hydrogel to porous BCP granule scaffold as a carrier role to improve translatability as well as enhance a cell attachment and proliferation behavior by highly porous interconnected structures and fast degradation rate. Single granular types scaffold (1mm diameter with 7 holes) which is fabricated by multi-pass extrusion method to generate micro-canal structures is composed of biphasic calcium phosphate (BCP). The HyA-Gel loaded BCP scaffold (HGBS) was characterized by SEM, porosity meter, and UTM machine for its microstructure, morphology, porosity and compressive strength. Optimized HGBS scaffolds show acceptable swelling and degradation characteristics. In vitro cytocompatibility studies were conducted by preosteoblasts (MC3T3-E1). The HGBS resulted in a significant increase in cell viability and proliferation compared to BS (raw BCP granule).In vivo investigation in a rabbit model after 4 weeks and 8 weeks of implantation showed full osteointegration of the scaffold with the native tissue, and formation of bone tissue within the pore network, as examined by microcomputed tomography analyses and histological staining. In histological section ensured a rapid new bone formation and a high rate collagen mineralization in HGBS scaffolds. In vivo expression of extracellular matrix proteins suggested that this HGBS can be used as an artificial bone substitute for stimulating damaged bone. |
| 저자 |
Omar Frauq1, Boram Kim2, Andrew R. Padalhin3, Byong-Taek Lee4
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| 소속 |
1Department of Regenerative Medicine, 2College of Medicine, 3Soonchunhyang Univ., 4Institute of Tissue Regeneration |
| 키워드 |
Hyaluronic acid; Gelatin; Hydrogel; Scaffold; Bone substitute; BCP; Bone tissue engineering
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| E-Mail |
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