| 학회 |
한국화학공학회 |
| 학술대회 |
2012년 봄 (04/25 ~ 04/27, 제주 ICC) |
| 권호 |
18권 1호, p.420 |
| 발표분야 |
생물화공 |
| 제목 |
capacitive aptasensor for label- free detection of the Nampt(PBEF/Visfatin) |
| 초록 |
Adipokines are signaling proteins secreted by adipose tissue in people with obesity. NAMPT (visfatin/PBEF) is one of adipokines that is related to various diseases, such as type 2 diabetes, chronic kidney dysfunction, inflammation and cancer. Therefore, in this study, a capacitive aptasensor was developed for the detection of NAMPT as a model adepokine. The detection of the aptamer-NAMPT complex formed on the surface of the electrode is based on dielectric properties, charge distribution and conductivity on the surface. This signal change was successfully measured by using gold interdigitated capacitor arrays onto which DNA aptamers were immobilized to detect various concentrations of NAMPT. Here, we demonstrate that specific ssDNA aptamer can be used as a powerful recognition molecule to the capaitor based label-free, non-invasive biosensors that has great potential for the early diagnosis of the adipokines related diseases. |
| 저자 |
박지웅1, Saravan Kallempudi2, 이수진1, 윤병수3, Yasar Gurbuz2, Javed H. Niazi2, 구만복1
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| 소속 |
1고려대, 2Sabanci Univ., 3AdipoGen |
| 키워드 |
aptamer; Nampt; biosensor
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| E-Mail |
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| 원문파일 |
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