| 학회 |
한국공업화학회 |
| 학술대회 |
2018년 가을 (10/31 ~ 11/02, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 |
22권 2호 |
| 발표분야 |
생물공학_포스터 |
| 제목 |
Expedite selection of astaxanthin-hyperproducing Haematococcus mutant via azide-based colorimetric assay combined with oil-based astaxanthin extraction |
| 초록 |
The aim of this work was to develop a new approach for simple and high-throughput selection of astaxanthinhyperproducing Haematococcus mutants through a sequential combination method of azide-based colorimetric assessment and oil-based astaxanthin quantification. Randomly mutagenized cells were spotted on solid culture medium containing 50µM of sodium azide to accelerate the biosynthesis of astaxanthin. After 3days, highlyinduced mutants were preliminarily isolated by visual inspection and their astaxanthin accumulations were rapidly quantified by soybean oil-based extraction method. On the whole, the selected mutants showed reduced vegetative growth rates but eventually exhibited higher astaxanthin productions than the parental strain owing to their improved inductive growths. |
| 저자 |
유병선1, 최홍일1, 장원석2, 심상준1
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| 소속 |
1고려대, 2한국지역난방공사 |
| 키워드 |
Haematococcus pluvialis; Autotrophic; astaxanthin production High-throughput screening Azide-based colorimetric assay
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| E-Mail |
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