| 학회 |
한국화학공학회 |
| 학술대회 |
2015년 봄 (04/22 ~ 04/24, 제주 ICC) |
| 권호 |
21권 1호, p.370 |
| 발표분야 |
생물화공 |
| 제목 |
Residue-Specific Dopa-Incorporated Engineered Mussel Bioadhesives |
| 초록 |
Dopa, which is a hydroxylated form of tyrosine, has been suggested to be the key factor for rapid and strong underwater adhesion. Mussel adhesive protein (MAP) is one example where Dopa chemistry is the core of their underwater adhesion with extremely high Dopa contents of ~10-25 mol%. The biosynthesis of recombinant MAPs in an E. coli system was a good approach but lack of Dopa in recombinant MAPs critically limited the underwater adhesion. Here, we explore the in vivo residue-specific Dopa incorporation into recombinant MAPs. Misaminoacylation of Dopa to tRNATyr by tyrosyl-tRNA synthetase allowed the quantitative replacement of tyrosine residues with a yield of over ~90 %, to create engineered MAPs in E. coli with a very high Dopa content. The Dopa-incorporated MAPs exhibited a superior surface adhesion and water resistance ability by assistance of Dopa-mediated interactions, and furthermore, showed underwater adhesive properties comparable to those of natural MAPs. These results propose promising use of Dopa-incorporated engineered MAPs as bioglues or adhesive hydrogels for practical underwater applications. |
| 저자 |
양병선1, Niraikulam Ayyadurai2, 윤형돈3, 최유성4, Jun Huang5, Qingye Lu5, Hongbo Zeng5, 황병희1, 차형준1
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| 소속 |
1포항공과대, 2Central Leather Research Institute, 3건국대, 4충남대, 5Univ. of Alberta |
| 키워드 |
biomimetic synthesis; Dopa; mussel adhesive proteins; noncanonical amino acids; underwater bioadhesive
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| E-Mail |
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| VOD |
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| 원문파일 |
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