| 초록 |
Various riboswitch-based genetic devices have been developed and applied to diverse fields. Since riboswitches should have distinct dose-response curves to meet the requirements for different applications, they have been redesigned by rational approaches. However, rational approach is applicable only to few riboswitches whose structure and regulatory mechanism are well understood. Therefore, we developed an evolutionary approach for riboswitch engineering that is independent of preliminary knowledge about the riboswitch. First, we obtained naringenin (NRN) aptamer library by systematic evolution of ligands by exponential enrichment, and constructed NRN riboswitch library. Then we performed enrichments by dual selection in low, medium and high NRN concentrations. We chose enrichment conditions by investigation of the relationship between expression level of tetA and resistance to selection pressures. After then, we will measure the dose-response curves of the enriched NRN riboswitches. |
