| 초록 |
Cancer cells have been found to differ from normal cells in several ways, but the most essential origin is from the regulation processes of gene expression. The upstream of this regulation is the complex interactions between an array of specific regulatory DNA sequences and proteins in gene transcription. Herein, we developed a new nanoplasmonic sensor to attain label-free, real-time, and cost-effective monitoring of the molecular interactions based on localized surface plasmon resonance (LSPR) by analysis of epigenetics-driven transcriptional repression of DNA methylation, binding efficiency of normal polymerase with mutated DNA, and then normal promoter with mutated protein obtained from cancer cells. The results were verified by highly consistent in vitro transcription efficiencies and closely met the theoretical and clinical expectations. |
